Functional endoscopic sinus surgery [FESS] in patients with hyperreactive airway disease

This Study was conducted as prospective study on 36 asthmatics with evidence of chronic rhinosinusitis. The aim of this study is to study the effect of chronic sinusitis and polypoidal sino-pathy on the lower airways and to elucidate the effect of functional endoscopic sinus surgery [FESS] on the hyperreactive airway disease and asthma associated with chronic sinusitis. The results of this Study 3 months and 6 months postoperativley showed a significant improvement of all major nasal symptoms [P<0.05] [Table 1] except for hyposmia which did not show the same improvement [P>0.05]. In both allergic and non allergic patients 3 months and 6 months postoperatively, there was a significant improvement in the mean asthma symptoms [P<0.05] [Table 2 and Fig. 1], asthma exacerbation [P<0.05] [Table 3, 4]. We concluded that, sinus disease is an aggravating and exacerbating factor in asthma rather than an inducer of asthma and this was proved by improvement of severe asthma markedly after elimination of the offending sinusitis by FESS and by observation of airway hyperresponsiveness in non asthmatic subjects with chronic sinusitis, and by the observation of improvement of airway hyperresponsiveness after FESS due to relief of nasal symptoms and sinus lesions

Impact of nursing intervention on viral hepatitis patient's knowledge affiliated in viral clinic assuit university hospital

The incidence of hepatitis in Egypt is annually increase because the lack of awareness about the disease. Hepatitis is a term covering a broad spectrum of diseases affecting the liver. Infectious forms of hepatitis are relatively common and can cause serious illness and even death. Prevention is the key solution for avoiding the consequences of this disease. The present study aims to impact of nursing intervention on viral hepatitis patient's knowledge affiliated in viral clinic Assuit University Hospital. The subjects of the study consists of 200 patients divided into two groups [100 case and 100 control] their age above 20 years diagnosed with viral hepatitis. Both sexes were included in this study. The results revealed that mean ages 35.78 +/- 10.49, 37.21 +/- 11.25. The majority 84%, 80% of patients in rural areas 87%, 89% male and 85%, 75% were married for the study and control groups respectively. 79%, 81% of patients discovered the disease accidentally. 94%, 89% of patients diagnosed through investigations. 100% of patients define of viral hepatitis correctly on post test for the study group but only 20% for control group. Regarding patients' knowledge about mode of transmission 99% of them through parenteral therapy 97%, 91% for the study and control groups had poor scores on pre test however in post test the majority of the study group 94% had very good score with a highly statistical significant difference. Most of the studied sample obtained low score during the pre test, and the results changed toward progression in the post test regarding patients' knowledge. As a results and in the light of the finding, the authors recommended that health education program should be carried out at a wide areas include rural and urban areas and also to health workers to understand the mode of transmission and precautions to minimize the risk of transmission. Hepatitis B vaccine should available for health care workers and allow a simple information through the media [radio and television]

relationship between acidic proteases and pathogenicity of the infective juveniles of heterorhabditid nematodes

The infective juveniles [IJs] of five Heterorhabditis bacuriophora strains, entomopathogenic nematodes, demonstrated great variation in their pathogenicity to three full grown larvae of Galleria mellonella, Spodoptera littolaris and Agrotis ipsilon after 48 hr post infection. The infectivity could be arranged in a descending order as follows: EB7 > EG8 >EB6 > EA2 > EG7. The mean mortality% of G. mellonella was increased by increasing the length of exposure time to each nematode strain individually. The activity of acidic proteases was evaluated in the five H. bacteriophora strains and ranged from 27.3 to 112.9 units/10 [5] IJs with specific activity ranged from 13.2-76.3 units/mg protein. Highest insect mortality% was recorded in the strains of proteolytic activity > 55.0 units/10 [5] IJs [EB7 and EG8] except H. bacteriophora EG7. The least proteolytic activity was estimated in the least invasive strain, H. bacteriophora EA2. The relationship between pathogenicity and proteolytic activity is discussed. Acidic proteases zymogram revealed variation among strains with respect to the number and the relative mobility of the bands. Total protein and infectivity were tracked during storage of IJs of H. bacteriophora EB7. The consumption of protein indicated that proteins might be significant energy reserves. Acidic proteases have been purified from II. bacteriophora EB7. Three isoenzymes of acidic proteases, PI, PII, PIII, were resolved by ion exchange chromatography. The isoenzyme PII was further purified by chromatography on Sephacryl S-200 and specific activity increased to 441.7 units/mg protein which represents 22.5 folds purification

Biochemical studies on citrullus vulgaris urease: stability and reactivation by some reducing agents

Watermelon [Citrullus vulgaris cv. "Giza 1"] urease was inhibited by the divalent metal ions. Mg2+, Ca2+, Ba2+ [10 mM] and Mn2+ [0.02 mM] partially inhibited the enzyme with 15.9, 28.8, 8.0 and 12.0% inhibition, respectively. The heavy metal ions inhibited the enzyme in the order of Hg2+ > Cd2+ > Zn2+ > Cu2+ > Co2+ > Ni2+. Thiols as reducing agents at low concentrations ranging from 0.0001 to 0.01 mM caused activation of the enzyme and preserved its activity. The enzyme was stored in 0.01 mM dithiothreitol and 0.1% penicillin- streptomycin. It was stable and free of bacterial contamination for 6 moths. The specification of this enzyme meets the prerequisites needed for preparation of diagnostic urea kit